Autofluorescence lifetime imaging with low photon budget

Jenu V. Chacko; Kevin w Eliceiri

doi:10.1117/12.2650275

6 March 2023 Autofluorescence lifetime imaging with low photon budget

Jenu V. Chacko, Kevin w Eliceiri

Proceedings Volume PC12373, Optical Biopsy XXI: Toward Real-Time Spectroscopic Imaging and Diagnosis; PC123730E (2023) https://doi.org/10.1117/12.2650275
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We present a multiphoton excitation based hyperdimensional imaging microscope (HDIM). We demonstrate how this system may be used for metabolic readouts, how to evaluate samples with a very low photon budget (<50 photons/pixel), and how to combine such systems with real-time visualizations. We demonstrate how this HDIM approach can help us with metabolic interpretations in two use cases: cancer cells using NAD(P)H and in plant cells using chlorophyll fluorescence.

Conference Presentation

Citation Download Citation

Jenu V. Chacko and Kevin w Eliceiri "Autofluorescence lifetime imaging with low photon budget", Proc. SPIE PC12373, Optical Biopsy XXI: Toward Real-Time Spectroscopic Imaging and Diagnosis, PC123730E (6 March 2023); https://doi.org/10.1117/12.2650275

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