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17 March 2023 Photoinitiator-free two-photon polymerization for 3D cell-scaffold fabrication
Atsushi Nakayama, Yasuaki Kumamoto, Menglu Li, Teng Li, Meiling Zheng, Katsumasa Fujita
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Proceedings Volume PC12412, Laser 3D Manufacturing X; PC124120Q (2023) https://doi.org/10.1117/12.2659621
Event: SPIE LASE, 2023, San Francisco, California, United States
Abstract
Here, we performed photoinitiator-free two-photon polymerization (TPP) to fabricate 3D cell micro-scaffolds. By using a visible femtosecond pulsed laser as excitation, TPP is induced in deep UV-absorbing moieties without the use of photo-initiators. We fabricated 3D micro-structures of biocompatible materials and glycidyl methacrylate protein without photoinitiators. By using Raman/Brillouin spectroscopy, we quantitatively investigated the molecular and mechanical properties of the 3D structures, which are important factors for cell functions and growth on scaffolds. We seeded cells on the 3D scaffolds and observed their attachment and proliferation without causing numerous cell deaths due to the absence of potentially cytotoxic photoinitiators.
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Atsushi Nakayama, Yasuaki Kumamoto, Menglu Li, Teng Li, Meiling Zheng, and Katsumasa Fujita "Photoinitiator-free two-photon polymerization for 3D cell-scaffold fabrication", Proc. SPIE PC12412, Laser 3D Manufacturing X, PC124120Q (17 March 2023); https://doi.org/10.1117/12.2659621
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KEYWORDS
Two photon polymerization
Polymerization
Nanofabrication
Nanolithography
Polymers
Proteins
Regenerative medicine
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