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We overview and further methods for using structured light to fabricate microscopic structures for optical trapping and cell study experiments through two-photon-photopolymerisation (2PP). We utilise a spatial light modulator (SLM) to correct wavefront distortions in-situ from gold nanoparticle reflections, which are shown to offer significant improvements for in-house nanofabrication. Aberration corrected 2PP offers a mechanism for using a simple, modified holographic optical tweezers apparatus to produce complex 3D micro-structures with as many as 50 independent foci. Custom-designed structures are used to induce and study the collective behaviour of bacteria allowing us to test otherwise simulated theories of cell dynamics.
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Declan Armstrong, Halina Rubinsztein-Dunlop, Timo Nieminen, Alexander Stilgoe, "Aberration corrected structured light for in-house fabrication of functional micro-structures," Proc. SPIE PC12436, Complex Light and Optical Forces XVII, PC124360R (17 March 2023); https://doi.org/10.1117/12.2657795