Detection and classification of ebola on microfluidic chips

Xue Lin; Xiangyu Jin; Yunqian Fan; Qin Huang; Yue Kou; Guo Zu; Shiguang Huang; Xiaosheng Liu; Guoliang Huang

doi:10.1117/12.2246154

31 October 2016 Detection and classification of ebola on microfluidic chips

Xue Lin, Xiangyu Jin, Yunqian Fan, Qin Huang, Yue Kou, Guo Zu, Shiguang Huang, Xiaosheng Liu, Guoliang Huang

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 100240B (2016) https://doi.org/10.1117/12.2246154
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

Point-of-care testing (POCT) for an infectious diseases is the prerequisite to control of the disease and limitation of its spread. A microfluidic chip for detection and classification of four strains of Ebola virus was developed and evaluated. This assay was based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and specific primers for Ebola Zaire virus, Ebola Sudan virus, Ebola Tai Forest virus and Ebola Bundibugyo virus were designed. The sensitivity of the microfluidic chip was under 10³ copies per milliliter, as determined by ten repeated tests. This assay is unique in its ability to enable diagnosis of the Ebola infections and simultaneous typing of Ebola virus on a single chip. It offers short reaction time, ease of use and high specificity. These features should enable POCT in remote area during outbreaks of Ebola virus.

Citation Download Citation

Xue Lin, Xiangyu Jin, Yunqian Fan, Qin Huang, Yue Kou, Guo Zu, Shiguang Huang, Xiaosheng Liu, and Guoliang Huang "Detection and classification of ebola on microfluidic chips", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100240B (31 October 2016); https://doi.org/10.1117/12.2246154

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