Quantitative optical biomarkers of lung cancer based intrinsic two-photon excited fluorescence signal

Jingwen Li; Zhenlin Zhan; Hongxin Lin; Ning Zuo; Xiaoqin Zhu; Shusen Xie; Jianxin Chen; Shuangmu Zhuo

doi:10.1117/12.2245730

31 October 2016 Quantitative optical biomarkers of lung cancer based intrinsic two-photon excited fluorescence signal

Jingwen Li, Zhenlin Zhan, Hongxin Lin, Ning Zuo, Xiaoqin Zhu, Shusen Xie, Jianxin Chen, Shuangmu Zhuo

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 100242I (2016) https://doi.org/10.1117/12.2245730
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

Alterations in the elastic fibers have been implicated in lung cancer. However, the label-free, microscopic imaging of elastic fibers in situ remains a major challenge. Here, we present the use of intrinsic two-photon excited fluorescence (TPEF) signal as a novel means for quantification of the elastic fibers in intact fresh human lung tissues. We obtained the TPEF images of elastic fibers from ex vivo the human lung tissues. We found that three features, including the elastic fibers area, the elastic fibers orientation, the elastic fibers structure, provide the quantitative identification of lung cancer and the direct visual cues for cancer versus non-cancer areas. These results suggest that the TPEF signal can be used as the label-free optical biomarkers for rapid clinical lung diagnosis and instant image-guided surgery.

Citation Download Citation

Jingwen Li, Zhenlin Zhan, Hongxin Lin, Ning Zuo, Xiaoqin Zhu, Shusen Xie, Jianxin Chen, and Shuangmu Zhuo "Quantitative optical biomarkers of lung cancer based intrinsic two-photon excited fluorescence signal", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100242I (31 October 2016); https://doi.org/10.1117/12.2245730

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