2D light scattering label-free cytometry using light-sheet illumination

Meiai Lin; Xuantao Su

doi:10.1117/12.2246158

31 October 2016 2D light scattering label-free cytometry using light-sheet illumination

Meiai Lin, Xuantao Su

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 100243I (2016) https://doi.org/10.1117/12.2246158
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

Two-dimensional (2D) light scattering cytometry has been demonstrated as an effective label-free technology for cell analysis. Here we develop the light-sheet illumination in 2D light scattering static cytometry. In our cytometer, a cylindrical lens is used to form the light-sheet for better excitation of the static cells under an inverted microscope. The thickness of the light-sheet measured in fluorescent solution is about 13 μm. Two-dimensional light scattering patterns of standard microspheres and yeast cells are obtained by using a complementary metal oxide semiconductor (CMOS) detector via a low numerical aperture (NA 0.4) optical objective. The experimental patterns characterized with fringe structures agree well with Mie theory simulated ones. Our results suggest that the light-sheet illumination is an effective excitation method for 2D light scattering label-free cytometry.

Citation Download Citation

Meiai Lin and Xuantao Su "2D light scattering label-free cytometry using light-sheet illumination", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100243I (31 October 2016); https://doi.org/10.1117/12.2246158

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