Study on choroidal neovascularization with anti-VEGF treatment in the mouse retina using optical coherence tomography angiography (Conference Presentation)

Jang Ryul Park; WooJhon Choi; Jaeryung Kim; Hye Kyong Hong; Yongjoo Kim; Yoonha Hwang; Sang Jun Park; Se Joon Woo; Pilhan Kim; Kyu Hyung Park; Gou Young Koh; Wang-Yuhl Oh

doi:10.1117/12.2251770

16 May 2017 Study on choroidal neovascularization with anti-VEGF treatment in the mouse retina using optical coherence tomography angiography (Conference Presentation)

Jang Ryul Park, WooJhon Choi, Jaeryung Kim, Hye Kyong Hong, Yongjoo Kim, Yoonha Hwang, Sang Jun Park, Se Joon Woo, Pilhan Kim, Kyu Hyung Park, Gou Young Koh, Wang-Yuhl Oh

Author Affiliations +

Proceedings Volume 10045, Ophthalmic Technologies XXVII; 100450G (2017) https://doi.org/10.1117/12.2251770
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

To understand the pathogenesis of ophthalmic disease, utilizing small animal models such as mouse is necessary because of their ease of maintenance and availability. For identifying pathophysiology and drug development of retinal diseases in mouse model, optical coherence tomography angiography (OCTA) is promising imaging modality visualizing not only microstructure but also microvasculature. In this study, we serially imaged 3D structure and angiography of laser-induced choroidal neovascularization (CNV) in the mouse retina with/without anti-VEGF treatment. Also, the volume changes of CNV and avascular region in choroid layer are measured for identifying effects of anti-VEGF. A lab-built high-speed OCTA prototype using the wavelength-swept laser centered at 1040 nm with 230 kHz A-scan rate acquired 3-D volumetric data consisted of 1024 x 1024 x 3 A-scans. The OCTA scanned 1.7 mm x 1.7 mm area around ONH. For obtaining angiography, amplitude decorrelation from 3 consecutive B-scans at each position was generated. Seven days after the laser photocoagulation at mouse retina for generation of the laser-induced CNV, intravitreal administration of Fc and VEGF-Trap was given in the therapeutic arm. The OCTA were performed at 6, 14, 21 and 35 days after laser photocoagulation. Vasculatures of inner retina, outer retina and choroid layers were separately visualized after RPE flattening and layer segmentation. To investigate therapeutic effects of anti-VEGF treatment, the relative area and volume of CNV in outer retina layer is measured. Also, total volume of avascular zone surrounding the laser injury site in choroid layer is also analyzed.

Conference Presentation

Citation Download Citation

Jang Ryul Park, WooJhon Choi, Jaeryung Kim, Hye Kyong Hong, Yongjoo Kim, Yoonha Hwang, Sang Jun Park, Se Joon Woo, Pilhan Kim, Kyu Hyung Park, Gou Young Koh, and Wang-Yuhl Oh "Study on choroidal neovascularization with anti-VEGF treatment in the mouse retina using optical coherence tomography angiography (Conference Presentation)", Proc. SPIE 10045, Ophthalmic Technologies XXVII, 100450G (16 May 2017); https://doi.org/10.1117/12.2251770

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Retina

Angiography

Optical coherence tomography

Laser coagulation

Visualization

3D modeling

Animal model studies

Show All Keywords

Keywords/Phrases

Search In:

Publication Years