Polarized Raman spectroscopic characterization of normal and oral cancer blood plasma

Rekha Pachaiappan; Aruna Prakasarao; Ganesan Singaravelu

doi:10.1117/12.2255600

23 March 2017 Polarized Raman spectroscopic characterization of normal and oral cancer blood plasma

Rekha Pachaiappan, Aruna Prakasarao, Ganesan Singaravelu

Proceedings Volume 10054, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XV; 100541F (2017) https://doi.org/10.1117/12.2255600
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

In India oral cancer ranks the top due to the habitual usage of tobacco in its various forms and remains the major burden. Hence priority is given for early diagnosis as it is the better solution for cure or to improve the survival rate. For the past three decades, optical spectroscopic techniques have shown its capacity in the discrimination of normal and malignant samples. Many research works have conventional Raman in the effective detection of cancer using the variations in bond vibrations of the molecules. However in addition polarized Raman provides the orientation and symmetry of biomolecules. If so can polarized Raman be the better choice than the conventional Raman in the detection of cancer? The present study aimed to found the answer for the above query. The conventional and polarized Raman spectra were acquired for the same set of blood plasma samples of normal subjects and oral malignant (OSCC) patients. Thus, obtained Raman spectral data were compared using linear discriminant analysis coupled with artificial neural network (LDA-ANN). The depolarization ratio of biomolecules such as antioxidant, amino acid, protein and nucleic acid bases present in blood plasma was proven to be the best attributes in the categorization of the groups. The polarized Raman results were promising in discriminating oral cancer blood plasma from that of normal blood plasma with improved efficiency. The results will be discussed in detail.

Citation Download Citation

Rekha Pachaiappan, Aruna Prakasarao, and Ganesan Singaravelu "Polarized Raman spectroscopic characterization of normal and oral cancer blood plasma", Proc. SPIE 10054, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XV, 100541F (23 March 2017); https://doi.org/10.1117/12.2255600

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available