Towards rapid prototyped convective microfluidic DNA amplification platform

Smrithi Ajit; Hemanth Mithun Praveen; Puneeth S. B.; Abhishek Dave; Bharat Sesham; K. N. Mohan; Sanket Goel

doi:10.1117/12.2257843

23 March 2017 Towards rapid prototyped convective microfluidic DNA amplification platform

Smrithi Ajit, Hemanth Mithun Praveen, Puneeth S. B., Abhishek Dave, Bharat Sesham, K. N. Mohan, Sanket Goel

Proceedings Volume 10061, Microfluidics, BioMEMS, and Medical Microsystems XV; 100610H (2017) https://doi.org/10.1117/12.2257843
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Today, Polymerase Chain Reaction (PCR) based DNA amplification plays an indispensable role in the field of biomedical research. Its inherent ability to exponentially amplify sample DNA has proven useful for the identification of virulent pathogens like those causing Multiple Drug-Resistant Tuberculosis (MDR-TB). The intervention of Microfluidics technology has revolutionized the concept of PCR from being a laborious and time consuming process into one that is faster, easily portable and capable of being multifunctional. The Microfluidics based PCR outweighs its traditional counterpart in terms of flexibility of varying reaction rate, operation simplicity, need of a fraction of volume and capability of being integrated with other functional elements. The scope of the present work involves the development of a real-time continuous flow microfluidic device, fabricated by 3D printing-governed rapid prototyping method, eventually leading to an automated and robust platform to process multiple DNA samples for detection of MDRTB-associated mutations. The thermal gradient characteristic to the PCR process is produced using peltier units appropriate to the microfluidic environment fully monitored and controlled by a low cost controller driven by a Data Acquisition System. The process efficiency achieved in the microfluidic environment in terms of output per cycle is expected to be on par with the traditional PCR and capable of earning the additional advantages of being faster and minimizing the handling.

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Smrithi Ajit, Hemanth Mithun Praveen, Puneeth S. B., Abhishek Dave, Bharat Sesham, K. N. Mohan, and Sanket Goel "Towards rapid prototyped convective microfluidic DNA amplification platform", Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 100610H (23 March 2017); https://doi.org/10.1117/12.2257843

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