Visualization of oxygen transportation in microcirculation by sidestream dark-field oximetry

Tomohiro Kurata; Minori Takahashi; Shigeto Oda; Hiroshi Kawahira; Takashi Ohnishi; Hideaki Haneishi

doi:10.1117/12.2253289

16 February 2017 Visualization of oxygen transportation in microcirculation by sidestream dark-field oximetry

Tomohiro Kurata, Minori Takahashi, Shigeto Oda, Hiroshi Kawahira, Takashi Ohnishi, Hideaki Haneishi

Proceedings Volume 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV; 1006813 (2017) https://doi.org/10.1117/12.2253289
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The sidestream dark-field (SDF) imaging allows direct visualization of red blood cells in microvessels near tissue surfaces. We have developed an image-based oximetry method using two-band images obtained by SDF imaging (SDF oximetry) and a trial SDF device with light-emitting diodes to obtain band images. In this study, we propose a technique of producing oxygen saturation (SO₂) maps from SDF images and perform animal experiments in vivo. To produce SO₂ maps, we use spectral analysis using two band images obtained with our SDF device. As an image processing, the combination of both the Hessian-based and pixel value-based techniques as blood vessel extraction from an SDF image is used. From the experiment with the surface of rat small intestines, we can produce SO₂ maps and find that the map represents arterioles and venules those were determined based on the blood ow from SDF images. Moreover, we find the variation of SO₂ along a blood vessel running direction.

Conference Presentation

Citation Download Citation

Tomohiro Kurata, Minori Takahashi, Shigeto Oda, Hiroshi Kawahira, Takashi Ohnishi, and Hideaki Haneishi "Visualization of oxygen transportation in microcirculation by sidestream dark-field oximetry", Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 1006813 (16 February 2017); https://doi.org/10.1117/12.2253289

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