In vivo multiphoton microscopy beyond 1 mm in the brain

David R. Miller; Flor A. Medina; Ahmed Hassan; Evan P. Perillo; Kristen Hagan; S. M. Shams Kazmi; Boris V. Zemelman; Andrew K. Dunn

doi:10.1117/12.2249949

21 February 2017 In vivo multiphoton microscopy beyond 1 mm in the brain

David R. Miller, Flor A. Medina, Ahmed Hassan, Evan P. Perillo, Kristen Hagan, S. M. Shams Kazmi, Boris V. Zemelman, Andrew K. Dunn

Author Affiliations +

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100690C (2017) https://doi.org/10.1117/12.2249949
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We perform high-resolution, non-invasive, in vivo deep-tissue imaging of the mouse neocortex using multiphoton microscopy with a high repetition rate optical parametric amplifier laser source tunable between λ=1,100 and 1,400 nm. We demonstrate an imaging depth of 1,200 μm in vasculature and 1,160 μm in neurons. We also demonstrate deep-tissue imaging using Indocyanine Green (ICG), which is FDA approved and a promising route to translate multiphoton microscopy to human applications.

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David R. Miller, Flor A. Medina, Ahmed Hassan, Evan P. Perillo, Kristen Hagan, S. M. Shams Kazmi, Boris V. Zemelman, and Andrew K. Dunn "In vivo multiphoton microscopy beyond 1 mm in the brain", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690C (21 February 2017); https://doi.org/10.1117/12.2249949

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