Label-free biomolecular characterization of human breast cancer tissue with stimulated Raman scattering (SRS) spectral imaging (Conference Presentation)

Fa-Ke F. Lu; David Calligaris; Yuanzhen Suo; Sandro Santagata; Alexandra J. Golby M.D.; X. Sunney Xie; Melissa A. Mallory; Mehra Golshan; Deborah A. Dillon; Nathalie Y. R. Agar

doi:10.1117/12.2253366

24 April 2017 Label-free biomolecular characterization of human breast cancer tissue with stimulated Raman scattering (SRS) spectral imaging (Conference Presentation)

Fa-Ke F. Lu, David Calligaris, Yuanzhen Suo, Sandro Santagata, Alexandra J. Golby M.D., X. Sunney Xie, Melissa A. Mallory, Mehra Golshan, Deborah A. Dillon, Nathalie Y. R. Agar

Author Affiliations +

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100690R (2017) https://doi.org/10.1117/12.2253366
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Stimulated Raman scattering (SRS) microscopy has been used for rapid label-free imaging of various biomolecules and drugs in living cells and tissues (Science, doi:10.1126/science.aaa8870). Our recent work has demonstrated that lipid and protein mapping of cancer tissue renders pathology-like images, providing essential histopathological information with subcellular resolution of the entire specimen (Cancer Research, doi: 10.1158/0008-5472.CAN-16-027). We have also established the first SRS imaging Atlas of human brain tumors (Harvard Dataverse, doi: (doi:10.7910/DVN/EZW4EK). SRS imaging of tissue could provide invaluable information for cancer diagnosis and surgical guidance in two aspects: rapid surgical pathology and quantitative biomolecular characterization. In this work, we present the use of SRS microscopy for characterization of a few essential biomolecules in breast cancer. Human breast cancer tissue specimens at the tumor core, tumor margin and normal area (5 cm away from the tumor) from surgical cases will be imaged with SRS at multiple Raman shifts, including the peaks for lipid, protein, blood (absorption), collagen, microcalcification (calcium phosphates and calcium oxalate) and carotenoids. Most of these Raman shifts have relatively strong Raman cross sections, which ensures high-quality and fast imaging. This proof-of-principle study is sought to demonstrate the feasibility and potential of SRS imaging for ambient diagnosis and surgical guidance of breast cancer.

Conference Presentation

Citation Download Citation

Fa-Ke F. Lu, David Calligaris, Yuanzhen Suo, Sandro Santagata, Alexandra J. Golby M.D., X. Sunney Xie, Melissa A. Mallory, Mehra Golshan, Deborah A. Dillon, and Nathalie Y. R. Agar "Label-free biomolecular characterization of human breast cancer tissue with stimulated Raman scattering (SRS) spectral imaging (Conference Presentation)", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690R (24 April 2017); https://doi.org/10.1117/12.2253366

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Tissues

Breast cancer

Tumors

Cancer

Raman scattering

Raman spectroscopy

Calcium

Show All Keywords

Keywords/Phrases

Search In:

Publication Years