Epi-direction detected multimodal imaging of an unstained mouse retina with a Yb-fiber laser

Gabrielle A. Murashova; Christopher A. Mancuso; Sanae Sakami; Krzysztof Palczewski; Grazyna Palczewska; Marcos Dantus

doi:10.1117/12.2252457

2 March 2017 Epi-direction detected multimodal imaging of an unstained mouse retina with a Yb-fiber laser

Gabrielle A. Murashova, Christopher A. Mancuso, Sanae Sakami, Krzysztof Palczewski, Grazyna Palczewska, Marcos Dantus

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100692K (2017) https://doi.org/10.1117/12.2252457
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

In this work, we present all epi-direction detected images of an unstained mouse retina using multiphoton microscopy with a sub-50 fs Yb-fiber laser centered at 1.07 μm. This wavelength regime is particularly important as the fundamental wavelength is transparent to the eye and the higher harmonics are above the DNA-damaging UV wavelengths. We present a characterization of the multimodal signals emitted from all the retinal layers, as well as from the choroid and the sclera. By characterizing native multiphoton signals from the retina, we move closer to having Yb-fiber considered for in vivo diagnosis of retinal disease through multiphoton microscopy as well as for corrective therapies.

Citation Download Citation

Gabrielle A. Murashova, Christopher A. Mancuso, Sanae Sakami, Krzysztof Palczewski, Grazyna Palczewska, and Marcos Dantus "Epi-direction detected multimodal imaging of an unstained mouse retina with a Yb-fiber laser", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100692K (2 March 2017); https://doi.org/10.1117/12.2252457

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