Confining Brownian motion of single nanoparticles in an ABELtrap

Maria Dienerowitz; Thomas Heitkamp; Thomas Gottschall; Jens Limpert; Michael Börsch

doi:10.1117/12.2250550

27 February 2017 Confining Brownian motion of single nanoparticles in an ABELtrap

Maria Dienerowitz, Thomas Heitkamp, Thomas Gottschall, Jens Limpert, Michael Börsch

Proceedings Volume 10120, Complex Light and Optical Forces XI; 1012017 (2017) https://doi.org/10.1117/12.2250550
Event: SPIE OPTO, 2017, San Francisco, California, United States

Abstract

Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusion through a confocal laser focus. We present an Anti-Brownian ELectrokinetic trap^1–7 (pioneered by A. E. Cohen and W. E. Moerner) to hold nanoparticles and individual F_oF₁-ATP synthase proteins in solution. We are interested in the conformational dynamics of this membrane-bound rotary motor protein that we monitor using single-molecule FRET. The ABELtrap is an active feedback system cancelling the nano-object’s Brownian motion by applying an electric field. We show how the induced electrokinetic forces confine the motion of nanoparticles and proteoliposomes to the centre of the trap.

Citation Download Citation

Maria Dienerowitz, Thomas Heitkamp, Thomas Gottschall, Jens Limpert, and Michael Börsch "Confining Brownian motion of single nanoparticles in an ABELtrap", Proc. SPIE 10120, Complex Light and Optical Forces XI, 1012017 (27 February 2017); https://doi.org/10.1117/12.2250550

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