The homogeneous and dual-medium cell’s refractive index decoupling method and entropy tomographic imaging

Z. D. Xin; Y. Y. Xu; Y. Ji; W. F. Jin; H. R. Zheng; L. Zhang; Y. W. Wang

doi:10.1117/12.2246701

19 October 2016 The homogeneous and dual-medium cell’s refractive index decoupling method and entropy tomographic imaging

Z. D. Xin, Y. Y. Xu, Y. Ji, W. F. Jin, H. R. Zheng, L. Zhang, Y. W. Wang

Author Affiliations +

Proceedings Volume 10152, High Power Lasers, High Energy Lasers, and Silicon-based Photonic Integration; 101520T (2016) https://doi.org/10.1117/12.2246701
Event: International Symposium on Optoelectronic Technology and Application 2016, 2016, Beijing, China

Abstract

In the paper, a decoupling method for homogeneous and dual-medium cells’ refractive index, and the entropy tomographic phase imaging method are proposed. Based on the decoupling method, the 3D morphology of sample can be obtained by the imaging method, which only needs two phase images of the cell. Thus the information about 3D refractive index distribution is given, and the 3D structure image of the model is reconstructed as well based on the relationship between the refractive index and thickness. In order to verify these methods, we set up the typical models after analysing the characteristic of blood cells, and the related orthogonal phase images are obtained by simulation experiment. Thus the 3D reconstructed structure images of the models are presented in this paper. Finally, the feasibility of this method is verified by simulating on a red blood cell and a monocyte model. The results show that subsurface imaging of samples can be achieved based on this method with a good accuracy.

Citation Download Citation

Z. D. Xin, Y. Y. Xu, Y. Ji, W. F. Jin, H. R. Zheng, L. Zhang, and Y. W. Wang "The homogeneous and dual-medium cell’s refractive index decoupling method and entropy tomographic imaging", Proc. SPIE 10152, High Power Lasers, High Energy Lasers, and Silicon-based Photonic Integration, 101520T (19 October 2016); https://doi.org/10.1117/12.2246701

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