PDI using nebulized indocyanine green for pneumonia treatment

Mariana C. Geralde; Giulia Kassab; Natalia M. Inada; Cristina Kurachi; Vanderlei S. Bagnato

doi:10.1117/12.2291987

14 February 2018 PDI using nebulized indocyanine green for pneumonia treatment

Mariana C. Geralde, Giulia Kassab, Natalia M. Inada, Cristina Kurachi, Vanderlei S. Bagnato

Proceedings Volume 10470, Endoscopic Microscopy XIII; 104700H (2018) https://doi.org/10.1117/12.2291987
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Infectious pneumonia is a major cause of morbidity/mortality, mainly due to the increasing rate of microorganisms resistant to antibiotics. Photodynamic Inactivation (PDI) is emerging as a promising treatment option, which effects are based on oxidative stress, targeting several biomolecules and probably preventing potential resistant strains. In previous studies, the in vitro inactivation of Streptococcus pneumoniae using indocyanine green (ICG) and infrared (IR) light source (780 nm) was successful, and achieving satisfactory reduction of colony-forming units (CFU/mL). In the present study, a proof-of-principle protocol was designed to treat lung infections by PDI using extracorporeal irradiation with a 780 nm laser device and nebulized ICG as photosensitizer. Balb/c mice were infected with S. pneumoniae and PDI was performed two days after infection using 800 μM of nebulized ICG and extracorporeal irradiation. Our results indicate that IR-extracorporeal PDI using nebulized ICG may be considered a potential pneumonia treatment, and pulmonary decontamination with PDI may be used as a single therapy or as an adjuvant for antibiotics.

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Citation Download Citation

Mariana C. Geralde, Giulia Kassab, Natalia M. Inada, Cristina Kurachi, and Vanderlei S. Bagnato "PDI using nebulized indocyanine green for pneumonia treatment", Proc. SPIE 10470, Endoscopic Microscopy XIII, 104700H (14 February 2018); https://doi.org/10.1117/12.2291987

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