Microcapillary imaging of lamina cribrosa in porcine eyes using photoacoustic microscopy

Mohesh Moothanchery; Thanadet Chuangsuwanich; Alvan Tsz Chung Yan; Leopold Schmetterer; Michael J. A. Girard; Manojit Pramanik

doi:10.1117/12.2287825

19 February 2018 Microcapillary imaging of lamina cribrosa in porcine eyes using photoacoustic microscopy

Mohesh Moothanchery, Thanadet Chuangsuwanich, Alvan Tsz Chung Yan, Leopold Schmetterer, Michael J. A. Girard, Manojit Pramanik

Author Affiliations +

Proceedings Volume 10494, Photons Plus Ultrasound: Imaging and Sensing 2018; 104945Q (2018) https://doi.org/10.1117/12.2287825
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

In order to understand the pathophysiology of glaucoma, Lamina cribrosa (LC) perfusion needs to be the subject of thorough investigation. It is currently difficult to obtain high resolution images of the embedded microcapillary network of the LC using conventional imaging techniques. In this study, an optical resolution photoacoustic microscopy (OR-PAM) system was used for imaging lamina cribrosa of an ex vivo porcine eye. Extrinsic contrast agent was used to perfuse the eye via its ciliary arteries. The OR-PAM system have a lateral resolution of 4 μm and an axial resolution of 30 μm. The high resolution system could able resolve a perfused LC microcapillary network to show vascular structure within the LC thickness. OR-PAM could be a promising imaging modality to study the LC perfusion and hence could be used to elucidate the hemodynamic aspect of glaucoma.

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Mohesh Moothanchery, Thanadet Chuangsuwanich, Alvan Tsz Chung Yan, Leopold Schmetterer, Michael J. A. Girard, and Manojit Pramanik "Microcapillary imaging of lamina cribrosa in porcine eyes using photoacoustic microscopy", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104945Q (19 February 2018); https://doi.org/10.1117/12.2287825

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