Evaluation of phototherapy in the differentiation of mesenchymal stem cells in the tissue repair of rats submitted to a hyperlipidemic diet

C. R. B. Oliveira; L. S. Santos; V. D. U. Silva; L. A. Vitória; T. T. Rodriguez; A. M. C. Marques; F. C. A. Xavier; L. Ramalho

doi:10.1117/12.2319049

12 April 2018 Evaluation of phototherapy in the differentiation of mesenchymal stem cells in the tissue repair of rats submitted to a hyperlipidemic diet

C. R. B. Oliveira, L. S. Santos, V. D. U. Silva , L. A. Vitória, T. T. Rodriguez, A. M. C. Marques, F. C. A. Xavier, L. Ramalho

Author Affiliations +

Proceedings Volume 10582, Laser Florence 2017: Advances in Laser Medicine; 105820A (2018) https://doi.org/10.1117/12.2319049
Event: Laser Florence 2017, 2017, Florence, Italy

Abstract

Obese people present a greater risk of developing other systemic diseases and comorbidities such as compromising the tissue repair process. Laser phototherapy can contribute to this repair by improving cellular functions, since stem cells may play an important role in repair due to their pluripotent potential. In this way, the influence of Laser Phototherapy (LP) was evaluated in the tissue repair of rats submitted to a hyperlipid diet through CD49 immunostaining for adipose stem cells. Forty-eight Wistar albinus rats were divided into two experimental groups: Standard Diet (SD) and Hyperlipid Diet (HD) for 20 weeks. After this period, excisional dorsal cutaneous wounds of 1 cm2 were made. The groups were subdivided into control and laser, the laser groups were irradiated (Diode Laser of Gallium and Aluminum Arsenide, λ660nm, 40mW, 6J / cm2) immediately after the surgery and every 48 hours. A group of rats were killed on day 7 and the other group on day 14 and the specimens processed by the immunohistochemical technique. The SD group presented antibodies marked with moderate to intense intensity, whereas in the HD group the weak staining for the time of 14 days prevailed. The irradiation protocol employed had no influence on the CD49 marker when compared to the control and irradiated groups over the same period. According to the methodology used and the results obtained it is concluded that laser light does not influence the recruitment of adipoderivative stem cells for the tissue repair process.

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C. R. B. Oliveira, L. S. Santos, V. D. U. Silva , L. A. Vitória, T. T. Rodriguez, A. M. C. Marques, F. C. A. Xavier, and L. Ramalho "Evaluation of phototherapy in the differentiation of mesenchymal stem cells in the tissue repair of rats submitted to a hyperlipidemic diet", Proc. SPIE 10582, Laser Florence 2017: Advances in Laser Medicine, 105820A (12 April 2018); https://doi.org/10.1117/12.2319049

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KEYWORDS

Tissues

Stem cells

Laser tissue interaction

Phototherapy

Laser dentistry

Tissue optics

Semiconductor lasers

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