Cellular refractive index comparison of various prostate cancer and noncancerous cell lines via photonic-crystal biosensor

Frank DeLuna; Melissa Cadena; Bingzhi Wang; Lu-Zhe Sun; Jing Yong Ye

doi:10.1117/12.2507505

4 March 2019 Cellular refractive index comparison of various prostate cancer and noncancerous cell lines via photonic-crystal biosensor

Frank DeLuna, Melissa Cadena, Bingzhi Wang, Lu-Zhe Sun, Jing Yong Ye

Author Affiliations +

Proceedings Volume 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII; 108810K (2019) https://doi.org/10.1117/12.2507505
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

The current clinical standard for mass screening of prostate cancer are prostate-specific antigen (PSA) biomarker assays. Unfortunately, the low specificity of PSA’s bioassays to prostate cancer leads to high false-positive rates, as such there is an urgent need for the development of a more specific detection system independent of PSA levels. In our previous research, we have successfully demonstrated, with the use of our Photonic-Crystal based biosensor in a Total-Internal- Reflection (PC-TIR) configuration, detection of prostate cancer (PC-3) cells against benign prostate hyperplasia (BPH-1) cells. The PC-TIR biosensor achieved detection of individual prostate cancer cells utilizing cellular refractive index (RI) as the only contrast parameter. To further study this methodology in vitro, we report a comprehensive study of the cellular RI’s of various prostate cancer and noncancerous cell lines (i.e. RWPE-1, BPH-1, PC-3, DU-145, and LNCaP) via reflectance spectroscopy and single-cell RI imaging utilizing the PC-TIR biosensor. Our study shows promising clinical potential in utilizing the PC-TIR biosensor system for the detection of prostate cancer against noncancerous prostate epithelial cells.

Conference Presentation

Citation Download Citation

Frank DeLuna, Melissa Cadena, Bingzhi Wang, Lu-Zhe Sun, and Jing Yong Ye "Cellular refractive index comparison of various prostate cancer and noncancerous cell lines via photonic-crystal biosensor", Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 108810K (4 March 2019); https://doi.org/10.1117/12.2507505

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