The features of temporal focusing multiphoton excitation fluorescence microscopy using the different excitation wavelength (Conference Presentation)

Fan-Ching Chien; Chun-Yu Lin; Chia-Yuan Chang; Chi-Hsiang Lien

doi:10.1117/12.2508141

4 March 2019 The features of temporal focusing multiphoton excitation fluorescence microscopy using the different excitation wavelength (Conference Presentation)

Fan-Ching Chien, Chun-Yu Lin, Chia-Yuan Chang, Chi-Hsiang Lien

Author Affiliations +

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108821L (2019) https://doi.org/10.1117/12.2508141
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Temporal focusing multiphoton excitation microscopy (TFMPEM) is insufficient to perform efficient excitation of fluorophores with the different two-photon absorption spectra in the multi fluorophores imaging via an illuminated configuration of fixed wavelength and polarization direction of the pulse laser because of the a diffraction device. In this study, we overcome and causes it is not useful for in the practical application and its development. In order to implement the temporal focusing microscope system could be able to sever for the different excitation wavelength range of 700-1000 nm, we work on the optical design of the temporal focusing an integration stage and scanning mirror with automatic tuning system for any excitation wavelength. The novel TFMPEM has successfully provided dynamic spectral absorption, 3D two-photon or SHG images with the original ability of the high frame rate.

Conference Presentation

Citation Download Citation

Fan-Ching Chien, Chun-Yu Lin, Chia-Yuan Chang, and Chi-Hsiang Lien "The features of temporal focusing multiphoton excitation fluorescence microscopy using the different excitation wavelength (Conference Presentation)", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108821L (4 March 2019); https://doi.org/10.1117/12.2508141

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