Label-free multispectral lifetime fluorescence to distinguish skin lesions

Renan A. Romano; Ramon G. T. Rosa; Javier A. Jo; Cristina Kurachi

doi:10.1117/12.2510498

4 March 2019 Label-free multispectral lifetime fluorescence to distinguish skin lesions

Renan A. Romano, Ramon G. T. Rosa, Javier A. Jo, Cristina Kurachi

Proceedings Volume 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019; 108902L (2019) https://doi.org/10.1117/12.2510498
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Skin lesions are the most common human cancer diseases, usually, is it diagnosed by clinical visual inspections followed by biopsy. Early detection of these diseases is critical, depending on an accurate and trained dermatologist and can increase the survival rate. Aiming for screening and early diagnose skin lesions many techniques are presented, however, optical techniques are highlighted since they are fast and noninvasive. In this context, fluorescence steady-state and lifetime imaging show potential by being able to image metabolic changes using endogenous contrast. Here it is demonstrated an in vivo label-free multispectral fluorescence lifetime imaging system to distinguish between two types of clinically similar lesions. A pulsed Nd:YAG laser emitting at 355 nm is used to excite the endogenous fluorophores and three channels of acquisition bands are used to imaging the skin. Preliminary results showed differences in the fluorescence lifetime between Bowen and Actinic Keratosis as well as the lesion and the skin around, demonstrating a potential tool to identify the lesion and its edges.

Citation Download Citation

Renan A. Romano, Ramon G. T. Rosa, Javier A. Jo, and Cristina Kurachi "Label-free multispectral lifetime fluorescence to distinguish skin lesions", Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 108902L (4 March 2019); https://doi.org/10.1117/12.2510498

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available