Quantitative Image Processing Through Phase Laser-Microscopy

Bruno Bianco; Alberto Diaspro; Tullio Tommasi

doi:10.1117/12.953189

1 May 1989 Quantitative Image Processing Through Phase Laser-Microscopy

Bruno Bianco, Alberto Diaspro, Tullio Tommasi

Proceedings Volume 1090, Medical Imaging III: Image Formation; (1989) https://doi.org/10.1117/12.953189
Event: Medical Imaging III, 1989, Newport Beach, CA, United States

Abstract

As a part of continuing investigation, this work aims to further characterize biological specimens, mantained in living conditions, through the use of non-invasive phase laser-microscopy. Dark-field and phase-contrast laser-microscopy and the relative signal processing techniques have been realized to recover the spatial distribution of the phase map of the object, obtainable after a proper processing. A simplified theory of image formation is discussed and some experimental results, on living CHO cells, are reported.

Citation Download Citation

Bruno Bianco, Alberto Diaspro, and Tullio Tommasi "Quantitative Image Processing Through Phase Laser-Microscopy", Proc. SPIE 1090, Medical Imaging III: Image Formation, (1 May 1989); https://doi.org/10.1117/12.953189

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