Three-dimensional particle localization with common-path digital holographic microscopy

Krisztian Neutsch; Lena Gö­ring; Marlon J. Tranelis; Martin R. Hofmann; Nils C. Gerhardt

doi:10.1117/12.2509448

1 March 2019 Three-dimensional particle localization with common-path digital holographic microscopy

Krisztian Neutsch, Lena Göring, Marlon J. Tranelis, Martin R. Hofmann, Nils C. Gerhardt

Proceedings Volume 10944, Practical Holography XXXIII: Displays, Materials, and Applications; 109440J (2019) https://doi.org/10.1117/12.2509448
Event: SPIE OPTO, 2019, San Francisco, California, United States

Abstract

In this paper, we present a common-path digital holographic microscope for 3D particle localization. Due to the common-path geometry, our setup is self-referencing, which reduces mechanical sensitivity to environmental disturbances. Thus, the system provides high stability and phase sensitivity down to 10nm. For system testing, different particles of varying size and material are distributed in a phantom material. Holograms are recorded by a camera and reconstructed with the angular spectrum method, phase unwrapping and Zernike polynomials. Numerical propagation of a single-shot hologram to multiple focal planes allow for optimal focusing on the sample. The resulting images show the different positions and distribution of particles.

Citation Download Citation

Krisztian Neutsch, Lena Göring, Marlon J. Tranelis, Martin R. Hofmann, and Nils C. Gerhardt "Three-dimensional particle localization with common-path digital holographic microscopy", Proc. SPIE 10944, Practical Holography XXXIII: Displays, Materials, and Applications, 109440J (1 March 2019); https://doi.org/10.1117/12.2509448

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