Diagnosis of lichen sclerosus based on multiphoton microscopy

Xiaomin Huang; Ying Huang; Liqin Zheng; Shu Wang; Lihang Lin; Xiaoqin Zhu; Jianxin Chen

doi:10.1117/12.2505859

15 November 2018 Diagnosis of lichen sclerosus based on multiphoton microscopy

Xiaomin Huang, Ying Huang, Liqin Zheng, Shu Wang, Lihang Lin, Xiaoqin Zhu, Jianxin Chen

Proceedings Volume 10964, Tenth International Conference on Information Optics and Photonics; 109642U (2018) https://doi.org/10.1117/12.2505859
Event: Tenth International Conference on Information Optics and Photonics (CIOP 2018), 2018, Beijing, China

Abstract

Lichen sclerosus (LS) is a chronic inflammatory dermatosis that caused substarntial discomfort and morbidity. Early diagnosis and treatment can prevent the occurrence of squamous cell carcinoma (SCC). Multiphoton microscopy (MPM) has the potential as an effective, noninvasive diagnostic tool for tissues imaging at a molecular level. The technique has several advantages including deeper penetration depth and minimal photo-toxicity and photo-bleaching compared with other microscopy techniques. In this work, MPM was expand to histological investigations, differentiating LS lesion from normal skin by imaging unstained histological sections without hematoxylin and eosin (H and E) staining. Our results present that MPM has the ability to identify the characteristics of LS including the changes of hyperkeratotic epidermis, homogenized collagen, and inflammatory cell infiltration. These tissue architecture details are in perfect agreement with the corresponding H and E-stained images. In addition, the results of collagen content show significant difference in normal skin and LS. The studies indicate that the MPM technique not only has the ability comparable to the H and E-stained images to distinguish between normal tissue and LS, but also can provides more comprehensive diagnostic information for the pathologist. With the advent of the clinical portability of MPM, this technique has the potential to be a powerful tool for diagnosing LS and monitoring the treatment response in vivo.

Citation Download Citation

Xiaomin Huang, Ying Huang, Liqin Zheng, Shu Wang, Lihang Lin, Xiaoqin Zhu, and Jianxin Chen "Diagnosis of lichen sclerosus based on multiphoton microscopy", Proc. SPIE 10964, Tenth International Conference on Information Optics and Photonics, 109642U (15 November 2018); https://doi.org/10.1117/12.2505859

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Skin

Collagen

Tissues

Second-harmonic generation

Multiphoton microscopy

Diagnostics

Optical fibers

Show All Keywords

Keywords/Phrases

Search In:

Publication Years