Identifying three different grades of human breast ductal carcinoma in situ using multiphoton microscopy

Zhong Chen; Tingfeng Shen; Liqin Zheng; Deyong Kang; Jianxin Chen

doi:10.1117/12.2548691

20 December 2019 Identifying three different grades of human breast ductal carcinoma in situ using multiphoton microscopy

Zhong Chen, Tingfeng Shen, Liqin Zheng, Deyong Kang, Jianxin Chen

Author Affiliations +

Proceedings Volume 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019); 112092W (2019) https://doi.org/10.1117/12.2548691
Event: Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 2019, Xi'an, China

Abstract

In recent years, the detection rate of ductal carcinoma in situ (DCIS) has been greatly increased due to the wide application of mammography. DCIS is a non-invasive neoplastic lesion that encompassed a heterogeneous group of lesions characterized by mild to severe atypia in ductal hyperplastic tumor cells. To facilitate the selection of appropriate treatments and to predict the risk of recurrence of DCIS, pathologists divide DCIS into three grades based primarily on nuclear grade (low grade, intermediate grade and high grade) and intraductal necrosis (comedo or punctate). Imaging and clinical examination failed to identify the grade of DCIS due to lack of adequate resolution. Clinically, the histopathological gold standard "hematoxylin and eosin-stained (H and E) sections" is used to identify the grade of DCIS. But, hematoxylin and eosin-stained (H and E) sections has several shortcomings, including time-consuming and complicated pathological procedures, labor intensive, and some subjective errors. Multiphoton microscopy (MPM) based on two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG), provides a label-free, low phototoxicity and high-resolution imaging method for label-free tissue samples. In this study, we used MPM to identify three different grades of human breast DCIS. Our results demonstrated that MPM can be rapidly used to identify three different grades of human breast DCIS on label-free tissue samples, via high-resolution imaging of intraductal proliferation of tumor cells, intraductal necrosis, and ductal basement membranes.

Citation Download Citation

Zhong Chen, Tingfeng Shen, Liqin Zheng, Deyong Kang, and Jianxin Chen "Identifying three different grades of human breast ductal carcinoma in situ using multiphoton microscopy", Proc. SPIE 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 112092W (20 December 2019); https://doi.org/10.1117/12.2548691

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Breast

Multiphoton microscopy

Second-harmonic generation

Tumors

Tissues

Collagen

Luminescence

Show All Keywords

Keywords/Phrases

Search In:

Publication Years