A paired-agent fluorescent molecular imaging strategy for quantifying antibody drug target engagement in in vivo window chamber xenograft models

Elif Kayaalp-Nalbant; Cody Rounds; Negar Sadeghipour; Boyu Meng; Margaret R. Folaron; Chandrika Haldar; Rendall R. Strawbridge; Kimberley S. Samkoe; Scott C. Davis; Kenneth M. Tichauer

doi:10.1117/12.2545182

19 February 2020 A paired-agent fluorescent molecular imaging strategy for quantifying antibody drug target engagement in in vivo window chamber xenograft models

Elif Kayaalp-Nalbant, Cody Rounds, Negar Sadeghipour, Boyu Meng, Margaret R. Folaron, Chandrika Haldar, Rendall R. Strawbridge, Kimberley S. Samkoe, Scott C. Davis, Kenneth M. Tichauer

Author Affiliations +

Proceedings Volume 11219, Visualizing and Quantifying Drug Distribution in Tissue IV; 112190G (2020) https://doi.org/10.1117/12.2545182
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

A paired-agent fluorescent molecular imaging strategy is presented as a method to measure drug target engagement in whole tumor imaging. The protocol involves dynamic imaging of a pair of targeted and control imaging agents prior to and following antibody therapy. Simulations demonstrated that antibody “drug target engagement” can be estimated within a 15%-error over a wide range of tumor physiology (blood flow, vascular permeability, target density) and antibody characteristics (affinity, binding rates). Experimental results demonstrated the first in vivo detection of binding site barrier, highlighting the potential for this methodology to provide novel insights in drug distribution/binding imaging.

Conference Presentation

Citation Download Citation

Elif Kayaalp-Nalbant, Cody Rounds, Negar Sadeghipour, Boyu Meng, Margaret R. Folaron, Chandrika Haldar, Rendall R. Strawbridge, Kimberley S. Samkoe, Scott C. Davis, and Kenneth M. Tichauer "A paired-agent fluorescent molecular imaging strategy for quantifying antibody drug target engagement in in vivo window chamber xenograft models", Proc. SPIE 11219, Visualizing and Quantifying Drug Distribution in Tissue IV, 112190G (19 February 2020); https://doi.org/10.1117/12.2545182

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available