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This talk will present a historical perspective of how multiphoton microscopy has revolutionized bioimaging and is continuing to opening up new modalities of imaging as well as the prospect of image-guided and light activated therapy .Major impetus has been provided by the development in three areas: 1) New biocompatible organic chromophores and their nanoformulations as well as inorganic nanocrystals with large optical nonlinearity; 2) Compact, user friendly and significantly lower cost ultra-short pulse lasers; 3) New designs of multiphoton microscope . They are described with examples from our work . Some Promising future directions will be discussed
Paras N. Prasad
"Evolution of multiphoton microscopy over three decades: Current perspectives and future directions (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124403 (9 March 2020); https://doi.org/10.1117/12.2549351
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Paras N. Prasad, "Evolution of multiphoton microscopy over three decades: Current perspectives and future directions (Conference Presentation)," Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124403 (9 March 2020); https://doi.org/10.1117/12.2549351