High speed 2-photon fluorescence lifetime imaging of protein-protein interactions with a swept array microscope (Conference Presentation)

Simon M. Ameer-Beg; Simon P. Poland; Thomas Kavanagh; James A. Levitt; Robert K. Henderson; Hanning Mai; Andrea Serio; Conor Treacy; Kelly O'Toole; Richard Walker

doi:10.1117/12.2544220

9 March 2020 High speed 2-photon fluorescence lifetime imaging of protein-protein interactions with a swept array microscope (Conference Presentation)

Simon M. Ameer-Beg, Simon P. Poland, Thomas Kavanagh, James A. Levitt, Robert K. Henderson, Hanning Mai, Andrea Serio, Conor Treacy, Kelly O'Toole, Richard Walker

Author Affiliations +

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 112441A (2020) https://doi.org/10.1117/12.2544220
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We report the development of a novel massively-parallelised high-speed multifocal FLIM platform with the ability to acquire data 1024 times faster than a conventional TCSPC system. We demonstrate the system performanceFRET imaging of the fluorescent protein biosensor PercivalHR in iPSC derived neurons to measure the dynamic concentration of ADP/ATP in live cells. The advantages and performance envelope of the system will be shown and the potential for further applications explored.

Conference Presentation

Citation Download Citation

Simon M. Ameer-Beg, Simon P. Poland, Thomas Kavanagh, James A. Levitt, Robert K. Henderson, Hanning Mai, Andrea Serio, Conor Treacy, Kelly O'Toole, and Richard Walker "High speed 2-photon fluorescence lifetime imaging of protein-protein interactions with a swept array microscope (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441A (9 March 2020); https://doi.org/10.1117/12.2544220

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KEYWORDS

Fluorescence lifetime imaging

Microscopes

Biosensors

Cameras

Imaging systems

Laser scanners

Live cell imaging

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