Multiphoton imaging of dye penetration dynamics in tissue sections

Po-Hang Tseng; Shu-Jen Chiang; Shean-Jen Chen; Chen-Yuan Dong

doi:10.1117/12.2545731

20 February 2020 Multiphoton imaging of dye penetration dynamics in tissue sections

Po-Hang Tseng, Shu-Jen Chiang, Shean-Jen Chen, Chen-Yuan Dong

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 1124428 (2020) https://doi.org/10.1117/12.2545731
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Rapid and in-depth labeling biological tissue samples is invaluable for studying tissue organization in three-dimensions. The current strategy in finding optimal labeling parameters for dye molecules through tissues often requires trial and error testing. Difficulty in establishing a standard approach is due to lack of information on the diffusion parameters of dye through tissues. In this study, we investigate the temporal progression of dye penetration in different tissue types. By the combination of multiphoton imaging of labeled tissues and application of the theoretical model of diffusion with well-defined boundary conditions, we aim to arrive at systematic parameters for optimizing dye penetration. Determination of the effective diffusion coefficient of the dyes used will then be useful for further application.

Citation Download Citation

Po-Hang Tseng, Shu-Jen Chiang, Shean-Jen Chen, and Chen-Yuan Dong "Multiphoton imaging of dye penetration dynamics in tissue sections", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124428 (20 February 2020); https://doi.org/10.1117/12.2545731

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KEYWORDS

Tissues

Diffusion

Molecules

Tissue optics

3D modeling

Imaging systems

Multiphoton microscopy

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