Wide-field label-free imaging of thick tissues using optical diffraction tomography (Conference Presentation)

Herve Hugonnet; Moosung Lee; Seungwoo Shin; Yongkeun Park

doi:10.1117/12.2551117

11 March 2020 Wide-field label-free imaging of thick tissues using optical diffraction tomography (Conference Presentation)

Herve Hugonnet, Moosung Lee, Seungwoo Shin, Yongkeun Park

Author Affiliations +

Proceedings Volume 11249, Quantitative Phase Imaging VI; 1124911 (2020) https://doi.org/10.1117/12.2551117
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Optical diffraction tomography (ODT) is a label-free three-dimensional (3D) microscopy technique allowing to retrieve the refractive-index distributions of optically translucent samples. Until recently, the histopathological applications of ODT have been impeded by the difficulty of imaging thick and wide tissues. Here, we demonstrate rapid, wide-field 3D ODT for imaging biological tissues. We designed a high-speed stitching ODT platform exploiting a digital micromirror device and a motorized stage. Developing a fast algorithmic framework that relieves the stitching artifacts and contrast loss due to multiple scattering, we demonstrate high-contrast 3D imaging of 100-m-thick, square-millimeter-wide pathological tissues at sub-micrometer resolution.

Conference Presentation

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Herve Hugonnet, Moosung Lee, Seungwoo Shin, and Yongkeun Park "Wide-field label-free imaging of thick tissues using optical diffraction tomography (Conference Presentation)", Proc. SPIE 11249, Quantitative Phase Imaging VI, 1124911 (11 March 2020); https://doi.org/10.1117/12.2551117

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KEYWORDS

Tissues

Diffraction

Optical tomography

Tissue optics

Tomography

Algorithm development

Reconstruction algorithms

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