Inkjet dispense SERS (ID-SERS) for quantitative analysis and bacterial detection

Fausto D'Apuzzo; Raghuvir N. Sengupta; Milo Overbay; Jason Aronoff; Anita Rogacs; Steven J. Barcelo

doi:10.1117/12.2545084

21 February 2020 Inkjet dispense SERS (ID-SERS) for quantitative analysis and bacterial detection

Fausto D'Apuzzo, Raghuvir N. Sengupta, Milo Overbay, Jason Aronoff, Anita Rogacs, Steven J. Barcelo

Proceedings Volume 11257, Plasmonics in Biology and Medicine XVII; 112570X (2020) https://doi.org/10.1117/12.2545084
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Surface-Enhanced Raman Scattering (SERS) has emerged as a powerful technique for sensitive detection of a wide variety of substances including drugs, pesticides, and biological molecules. Despite its potential as a broadly applicable detection strategy, quantitative studies using SERS have been hindered by substrate performance variability. We recently reported an in situ calibration method that merges inkjet dispense with SERS (ID-SERS), yielding quantitative measurements with Relative Standard Deviation (RSD) values below 2%. In this work, we provide a conceptual review of ID-SERS, highlighting the advantages of using inkjet to pattern microdroplets (28 pL) on the surface of a single SERS sensor and produce standard curves on a single SERS sensor. We subsequently explore the application potential of IDSERS by producing ID-SERS response curves for adenine and pyocyanin, two well-known metabolites that are biomarkers for bacterial detection. We then report preliminary results that suggest that ID-SERS can be harnessed to detect time-dependent changes in the spectra of metabolites released by bacteria under nutrient-deprived conditions. The ability to use ID-SERS to monitor the time-evolution in the SERS spectra of nutrient-deprived bacteria has implications for developing a SERS-based method for antibiotic susceptibility testing. More generally, we believe that the benefits afforded by ID-SERS will facilitate the development of reproducible plasmonic sensing for real-world applications.

Citation Download Citation

Fausto D'Apuzzo, Raghuvir N. Sengupta, Milo Overbay, Jason Aronoff, Anita Rogacs, and Steven J. Barcelo "Inkjet dispense SERS (ID-SERS) for quantitative analysis and bacterial detection", Proc. SPIE 11257, Plasmonics in Biology and Medicine XVII, 112570X (21 February 2020); https://doi.org/10.1117/12.2545084

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