Single low-NA objective counterpropagating optical traps enabled by 3D-printed mirrors (Conference Presentation)

Andrea Bertoncini; Gheorghe Cojoc; Jochen Guck; Carlo Liberale

doi:10.1117/12.2545010

9 March 2020 Single low-NA objective counterpropagating optical traps enabled by 3D-printed mirrors (Conference Presentation)

Andrea Bertoncini, Gheorghe Cojoc, Jochen Guck, Carlo Liberale

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Proceedings Volume 11297, Complex Light and Optical Forces XIV; 112970H (2020) https://doi.org/10.1117/12.2545010
Event: SPIE OPTO, 2020, San Francisco, California, United States

Abstract

We show a dynamic counterpropagating optical trapping scheme based on a single low-NA objective and two right-angle prism mirrors µ-3D printed on a glass coverslip. Multiple beams are created with a Spatial Light Modulator and redirected to face each other by the mirrors. The key advantages of this approach are the simple alignment, the long working distance that allows trapping of large samples, the straightforward compatibility with other advanced microscopies and the intrinsic side-view of the trapped object. We demonstrate the viability of our approach by performing trapping and 3D manipulation of dielectric beads and cells.

Conference Presentation

Citation Download Citation

Andrea Bertoncini, Gheorghe Cojoc, Jochen Guck, and Carlo Liberale "Single low-NA objective counterpropagating optical traps enabled by 3D-printed mirrors (Conference Presentation)", Proc. SPIE 11297, Complex Light and Optical Forces XIV, 112970H (9 March 2020); https://doi.org/10.1117/12.2545010

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KEYWORDS

Mirrors

Objectives

Optical tweezers

Spatial light modulators

Lenses

Optical fibers

Optical manipulation

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