Optical biopsy of skin cancer based on Raman and fluorescence spectroscopy

Yulia A. Khristoforova; Ivan A. Bratchenko; Lyudmila A. Bratchenko; Oleg O. Myakinin; Dmitry N. Artemyev; Alexandr A. Moryatov; Sergey V. Kozlov; Ekaterina G. Borisova; Tsanislava I. Genova; Petranka P. Troyanova; Valery P. Zakharov

doi:10.1117/12.2581594

17 November 2020 Optical biopsy of skin cancer based on Raman and fluorescence spectroscopy

Yulia A. Khristoforova, Ivan A. Bratchenko, Lyudmila A. Bratchenko, Oleg O. Myakinin, Dmitry N. Artemyev, Alexandr A. Moryatov, Sergey V. Kozlov, Ekaterina G. Borisova, Tsanislava I. Genova, Petranka P. Troyanova, Valery P. Zakharov

Author Affiliations +

Proceedings Volume 11582, Fourth International Conference on Terahertz and Microwave Radiation: Generation, Detection, and Applications; 115821K (2020) https://doi.org/10.1117/12.2581594
Event: Fourth International Conference on Terahertz and Microwave Radiation: Generation, Detection, and Applications, 2020, Tomsk, Russian Federation

Abstract

This paper presents the application of the optical methods of Raman spectroscopy and autofluorescence analysis for studying of the skin diagnostic features. Benign and malignant skin tumors from 89 patients were measured using portable spectroscopic system with near-infrared 785 nm laser excitation. Classifications of the different skin tumors were made using partial least squares methods with discriminant analysis in two tasks: melanoma from other malignant skin tumors (basal cell carcinoma and squamous cell carcinoma) with 0.95 sensitivity and 0.86 specificity and melanoma from pigmented nevus with 0.68 sensitivity and 0.78 specificity. The main findings establish that combined analysis of the autofluorescence and Raman spectral signatures of the different skin tumors are more effective in comparison with using of the only Raman spectroscopy. Also, results of classifications show the tumor spectra contain most useful information rather than tumor spectra normalized to the corresponding normal skin spectra.

Citation Download Citation

Yulia A. Khristoforova, Ivan A. Bratchenko, Lyudmila A. Bratchenko, Oleg O. Myakinin, Dmitry N. Artemyev, Alexandr A. Moryatov, Sergey V. Kozlov, Ekaterina G. Borisova, Tsanislava I. Genova, Petranka P. Troyanova, and Valery P. Zakharov "Optical biopsy of skin cancer based on Raman and fluorescence spectroscopy", Proc. SPIE 11582, Fourth International Conference on Terahertz and Microwave Radiation: Generation, Detection, and Applications, 115821K (17 November 2020); https://doi.org/10.1117/12.2581594

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available