Optical Sectioning With The Scanning Slit Confocal Microscope: Applications In Ophthalmology And Ear Research

Charles J. Koester; Shyam M. Khanna; Robert B. Tackaberry

doi:10.1117/12.962720

22 December 1989 Optical Sectioning With The Scanning Slit Confocal Microscope: Applications In Ophthalmology And Ear Research

Charles J. Koester, Shyam M. Khanna, Robert B. Tackaberry

Author Affiliations +

Proceedings Volume 1161, New Methods in Microscopy and Low Light Imaging; (1989) https://doi.org/10.1117/12.962720
Event: 33rd Annual Technical Symposium, 1989, San Diego, United States

Abstract

Confocal slits offer certain advantages over confocal pinholes for optical sectioning microscopy, particularly with non-laser illumination. One-dimensional scanning simplifies the instrumentation and does not generate scan lines in the image. Slit widths can be adjusted during an experiment, in order to optimize the optical section depth and image illuminance. The optical sectioning effect provided by the out-of-focus effect can be enhanced by separating the illuminating and imaging light paths through the objective lens and within the specimen except for the region of the focal plane. Cellular structures are observed in vivo in the cornea and in the cochlea.

Citation Download Citation

Charles J. Koester, Shyam M. Khanna, and Robert B. Tackaberry "Optical Sectioning With The Scanning Slit Confocal Microscope: Applications In Ophthalmology And Ear Research", Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); https://doi.org/10.1117/12.962720

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