Three-Dimensional Reconstruction Of Noisy Confocal Scanning Microscope Images

Jose-Angel Conchello; Eric W. Hansen

doi:10.1117/12.962710

22 December 1989 Three-Dimensional Reconstruction Of Noisy Confocal Scanning Microscope Images

Jose-Angel Conchello, Eric W. Hansen

Proceedings Volume 1161, New Methods in Microscopy and Low Light Imaging; (1989) https://doi.org/10.1117/12.962710
Event: 33rd Annual Technical Symposium, 1989, San Diego, United States

Abstract

Confocal scanning microscopes have improved depth discrimination over conventional microscopes. The out-of-focus contributions in a through-focus series of images are greatly reduced by the confocal geometry but not completely removed. Simulations have shown that posteriori iterative constrained deconvolution can produce depth discrimination equal to the lateral diffraction limit. However, such deterministic algorithms perform poorly when the optical sections are noisy, such as occurs in ultralow-light fluorescence microscopy. In this paper we present a stochastic approach to 3-D reconstruction and discuss the relationship of the microscope's imaging properties to the postprocessing task.

Citation Download Citation

Jose-Angel Conchello and Eric W. Hansen "Three-Dimensional Reconstruction Of Noisy Confocal Scanning Microscope Images", Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); https://doi.org/10.1117/12.962710

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