Ex vivo metabolic imaging of tumor samples from patients by FLIM

Maria M. Lukina; Lubov E. Shimolina; Varvara V. Dudenkova; Alena I. Gavrina; Vladimir E. Zagainov; Dmitriy V. Komarov; Konstantin S. Yashin; Igor A. Medyanik; Elena V. Zagaynova M.D.; Marina V. Shirmanova

doi:10.1117/12.2579211

5 March 2021 Ex vivo metabolic imaging of tumor samples from patients by FLIM

Maria M. Lukina, Lubov E. Shimolina, Varvara V. Dudenkova, Alena I. Gavrina, Vladimir E. Zagainov, Dmitriy V. Komarov, Konstantin S. Yashin, Igor A. Medyanik, Elena V. Zagaynova M.D., Marina V. Shirmanova

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Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116480N (2021) https://doi.org/10.1117/12.2579211
Event: SPIE BiOS, 2021, Online Only

Abstract

The aim of our work was to investigate energy metabolism of patient’s tumor ex vivo using fluorescence lifetime imaging (FLIM). Tumor metabolism was examined by detecting the fluorescence lifetime of metabolic cofactor NAD(P)H. In glioma tumor samples was shown a different energy metabolism with white matter. Colorectal tumors displayed marked intertumor heterogeneity and shift towards oxidative energy metabolism. Thereby, the possibility of assessing the energy metabolism in postoperative tumor samples of patients was shown.

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Maria M. Lukina, Lubov E. Shimolina, Varvara V. Dudenkova, Alena I. Gavrina, Vladimir E. Zagainov, Dmitriy V. Komarov, Konstantin S. Yashin, Igor A. Medyanik, Elena V. Zagaynova M.D., and Marina V. Shirmanova "Ex vivo metabolic imaging of tumor samples from patients by FLIM", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480N (5 March 2021); https://doi.org/10.1117/12.2579211

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