Rapid autofocusing for dynamic observation exploiting holographic polarization microscope

Leiping Che; Wen Xiao; Pietro Ferraro; Feng Pan

doi:10.1117/12.2592794

20 June 2021 Rapid autofocusing for dynamic observation exploiting holographic polarization microscope

Leiping Che, Wen Xiao, Pietro Ferraro, Feng Pan

Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 117860P (2021) https://doi.org/10.1117/12.2592794
Event: SPIE Optical Metrology, 2021, Online Only

Abstract

We proposed a rapid autofocusing method exploiting holographic polarization microscope, which could determine the refocusing distance without multiple reconstructions and complex network training. The defocus distance of the object is calculated rapidly by identifying the separation distance of the two defocus images of the object and using the linear relation between the defocus distance of the object and the separation distance of the two defocus images of the object. The linear relationship is obtained by numerical propagation and linear fitting. Last, we have demonstrated the effectiveness of the proposed method by reconstructing the in-focus images of flowing cells. We believe that our proposed method could be a powerful tool for dynamic observation and extend the application of holographic polarization microscope.

Conference Presentation

Citation Download Citation

Leiping Che, Wen Xiao, Pietro Ferraro, and Feng Pan "Rapid autofocusing for dynamic observation exploiting holographic polarization microscope", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 117860P (20 June 2021); https://doi.org/10.1117/12.2592794

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