High-sensitivity measurements of autofluorescence using optical interference filters

Jeremy Grace; Samad Edlou; Joseph Foss; Craig Hodgson; Jean-Philippe Rheault; Jake Rosvold; Kurt Sieber; Sarah Walters

doi:10.1117/12.2602380

28 October 2021 High-sensitivity measurements of autofluorescence using optical interference filters

Jeremy Grace, Samad Edlou, Joseph Foss, Craig Hodgson, Jean-Philippe Rheault, Jake Rosvold, Kurt Sieber, Sarah Walters

Proceedings Volume 11889, Optifab 2021; 118890C (2021) https://doi.org/10.1117/12.2602380
Event: SPIE Optifab, 2021, Rochester, New York, United States

Abstract

We present a straightforward approach to making sensitive fluorescence measurements using a chopped laser and phase-lock detection scheme. By using high-quality optical filters in the excitation and detection paths, the trade-off between spectral information and ultimate sensitivity is controlled by the choice of filter edge locations and bandwidths. Using this approach, we demonstrate a sensitivity of ~1 pW optical power of detected fluorescence over a bandwidth of ~125 nm centered at 505 nm using a 405 nm diode laser as an excitation source. We present the details of the measurement technique and discuss its value in selection of optical materials for fluorescence-based analytical systems.

Conference Presentation

Citation Download Citation

Jeremy Grace, Samad Edlou, Joseph Foss, Craig Hodgson, Jean-Philippe Rheault, Jake Rosvold, Kurt Sieber, and Sarah Walters "High-sensitivity measurements of autofluorescence using optical interference filters", Proc. SPIE 11889, Optifab 2021, 118890C (28 October 2021); https://doi.org/10.1117/12.2602380

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