Presentation + Paper
2 March 2022 Multicolor stimulated Raman scattering microscopy and its applications
Author Affiliations +
Abstract
Stimulated Raman scattering (SRS) microscopy allows for sensitive molecular vibrational imaging and is opening up a variety of biomedical applications. To fully utilize the capability of SRS, we developed a multicolor SRS microscope, which is capable of acquiring SRS images at the video rate, while the wavenumber can be changed in a frame-by-frame manner. Also our system is integrated with a confocal fluorescence microscope. I will introduce various vibrational imaging applications such as label-free imaging, supermultiplex imaging, metabolic imaging, and so on. We also describe functional Raman probes, which will further broaden the applications of SRS microscopy.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yasuyuki Ozeki "Multicolor stimulated Raman scattering microscopy and its applications", Proc. SPIE 11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, 1195709 (2 March 2022); https://doi.org/10.1117/12.2615280
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KEYWORDS
Microscopes

Imaging systems

Oscillators

Microscopy

Raman scattering

Wavelength tuning

Boron

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