Fourfold resolution enhancement in quantitative phase microscopy through second harmonic generation and structured illumination

Nansen Zhou; Andreas Zheng; Renjie Zhou

doi:10.1117/12.2610450

2 March 2022 Fourfold resolution enhancement in quantitative phase microscopy through second harmonic generation and structured illumination

Nansen Zhou, Andreas Zheng, Renjie Zhou

Author Affiliations +

Proceedings Volume 11970, Quantitative Phase Imaging VIII; 1197003 (2022) https://doi.org/10.1117/12.2610450
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We propose quantitative phase microscopy (QPM) with second-harmonic generation (SHG) and structured illumination, namely S²QPM, that can achieve fourfold resolution enhancement compared with normal incident illumination. The wave propagation and reconstruction model in S²QPM are provided from solving the inhomogeneous nonlinear Helmholtz equation in the k-space. Our derived physical model is accurate and does not involve far-field approximations. To validate our theoretical model, a Siemens star phase object has been reconstructed under simulated experimental conditions. We envision that S²QPM may potentially open more exciting applications in label-free bioimaging and material characterization. .

Conference Presentation

Citation Download Citation

Nansen Zhou, Andreas Zheng, and Renjie Zhou "Fourfold resolution enhancement in quantitative phase microscopy through second harmonic generation and structured illumination", Proc. SPIE 11970, Quantitative Phase Imaging VIII, 1197003 (2 March 2022); https://doi.org/10.1117/12.2610450

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