Porphyrin photosensitivity in cell lines expressing a heat-resistant phenotype

Charles J. Gomer; Natalie Rucker; Sam Wong

doi:10.1117/12.17663

1 July 1990 Porphyrin photosensitivity in cell lines expressing a heat-resistant phenotype

Charles J. Gomer, Natalie Rucker, Sam Wong

Proceedings Volume 1203, Photodynamic Therapy: Mechanisms II; (1990) https://doi.org/10.1117/12.17663
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

In-vitro sensitivity to porphyrin mediated photodynamic therapy (PDT) has been examined in cell lines resistant to hyperthermia. Parental (HA-i) and heat resistant (3012) Chinese hamster fibroblasts as well as parental (RIF-i) and temperature resistant (TR-4, TR-5 and TR-iO) mouse radiation-induced fibrosarcoma cells were evaluated for thermal and PDT sensitivity. Quantitative survival curves were generated and porphyrin uptake properties were obtained for all cell lines. Significant resistance to hyperthermia (450C for varying exposure periods) was documented for the 3012 and TR cell strains when compared to 'the parent lines. However, normal and heat resistant clones exhibited comparable levels of porphyrin uptake and photosensitivity. Our results indicate that cross resistance between hyperthermia and PDT is not observed and that members of the 70 kD heat shock protein family (which are elevated in the thermal resistant cells and may be associated with the heat resistant phenotype) do not play a significant role in modulating PDT sensitivity. Mechanisms of in-vitro cytotoxicity appear to be different for PDT and hyperthermia even though possible subcellular targets (such as the plasma membrane) and types of damage (protein denaturation) may be similar for the two modalities.

Citation Download Citation

Charles J. Gomer, Natalie Rucker, and Sam Wong "Porphyrin photosensitivity in cell lines expressing a heat-resistant phenotype", Proc. SPIE 1203, Photodynamic Therapy: Mechanisms II, (1 July 1990); https://doi.org/10.1117/12.17663

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