Three-dimensional imaging of porcine joints down to the subcellular level

Georg Schulz; Andrea Barbero; Francine Wolf; Griffin Rodgers; Christine Tanner; Timm Weitkamp; Marcus Mumme; Marta Morawska; Daniel Beer; Bert Müller

doi:10.1117/12.2635223

16 November 2022 Three-dimensional imaging of porcine joints down to the subcellular level

Georg Schulz, Andrea Barbero, Francine Wolf, Griffin Rodgers, Christine Tanner, Timm Weitkamp, Marcus Mumme, Marta Morawska, Daniel Beer, Bert Müller

Author Affiliations +

Proceedings Volume 12242, Developments in X-Ray Tomography XIV; 1224215 (2022) https://doi.org/10.1117/12.2635223
Event: SPIE Optical Engineering + Applications, 2022, San Diego, California, United States

Abstract

Joint tissues consist of trabecular and cortical bone as well as calcified and hyaline cartilage, which presents a challenge for hard X-ray-based visualization on the sub-cellular level due to the wide range of local X-ray absorption values. The density of the calcified tissues requires rather high photon energy, which often leads to insufficient contrast within the cartilage and impedes the visualization of individual biological cells. Decalcification of the tissues reduces the total and local X-ray absorption values and allows for selecting a lower photon energy. Further contrast enhancement can be achieved by ethanol fixation and paraffin tissue embedding. In this study, we (i) searched for an appropriate visualization method to investigate lesions generated by a laser osteotome and (ii) visualized a decalcified porcine joint after ethanol fixation and subsequent paraffin embedding using laboratory- and synchrotron radiation-based microtomography. The experiments at the ANATOMIX beamline of Synchrotron SOLEIL were performed in off-axis scan mode with a pixel size of 1.3 µm. Individual cells in all layers of the joint could be made visible and the effect of ethanol fixation and paraffin embedding demonstrated.

Conference Presentation

Citation Download Citation

Georg Schulz, Andrea Barbero, Francine Wolf, Griffin Rodgers, Christine Tanner, Timm Weitkamp, Marcus Mumme, Marta Morawska, Daniel Beer, and Bert Müller "Three-dimensional imaging of porcine joints down to the subcellular level", Proc. SPIE 12242, Developments in X-Ray Tomography XIV, 1224215 (16 November 2022); https://doi.org/10.1117/12.2635223

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available