High Performance Liquid Chromatography-Diode Array Detection (HPLC-DAD) method for the determination of three components in the leaves of Stauntonia Brachyanthera from different regions

Lanqing Li; Zhenmin Zhang; Genyun Tang; Bin Huang

doi:10.1117/12.2657097

18 October 2022 High Performance Liquid Chromatography-Diode Array Detection (HPLC-DAD) method for the determination of three components in the leaves of Stauntonia Brachyanthera from different regions

Lanqing Li, Zhenmin Zhang, Genyun Tang, Bin Huang

Author Affiliations +

Proceedings Volume 12349, International Conference on Agri-Photonics and Smart Agricultural Sensing Technologies (ICASAST 2022); 123490S (2022) https://doi.org/10.1117/12.2657097
Event: International Conference on Agri-Photonics and Smart Agricultural Sensing Technologies (ICASAST 2022), 2022, Zhengzhou, China

Abstract

The main objective of this study was to establish a method for determining the content of orientin, calceolarioside B, kaempferol-3-O-rutinoside in Stauntonia Brachyanthera, and to compare the quality differences of Stauntonia Brachyanthera in different regions. The determination was performed on a Diamonsil®C18 column (4.6 mm × 250 mm, 5 μm) with acetonitrile-0.2% formic acid water as mobile phase, gradient elution, detection wavelength at 340nm, and column at room temperature. Orientin, calceolarioside B, kaempferol-3-O-rutinoside have a good linear relationship with peak area integration values. The regression equation is y = 1700.9x + 2.1473 (R² = 0.9997), y = 1131.5x + 5.8228 (R² = 0.9992), y = 1602x + 2.1948 (R² = 0.9993). The average dosing recovery rates were 98.6% (RSD = 1.76%), 102.6% (RSD = 1.88%), 99.6% (RSD = 1.25%). The High Performance Liquid Chromatography-Diode Array Detection (HPLC-DAD) method is suitable for the rapid determination of three components in the leaves of the Stauntonia Brachyanthera. The three components were different in the leaves of Stauntonia Brachyanthera from different regions, and the comprehensive analysis of Hongjiang has the highest content, which may be related to the picking period, soil quality, climate and other conditions.

Citation Download Citation

Lanqing Li, Zhenmin Zhang, Genyun Tang, and Bin Huang "High Performance Liquid Chromatography-Diode Array Detection (HPLC-DAD) method for the determination of three components in the leaves of Stauntonia Brachyanthera from different regions", Proc. SPIE 12349, International Conference on Agri-Photonics and Smart Agricultural Sensing Technologies (ICASAST 2022), 123490S (18 October 2022); https://doi.org/10.1117/12.2657097

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Detector arrays

Medicine

Liver

Quantitative analysis

Sensors

Standards development

Testing and analysis

Show All Keywords

Keywords/Phrases

Search In:

Publication Years