Quantitative oblique back-illumination microscopy with enhanced nuclear phase contrast using acetic acid

Zhe Guang; Amunet Jacobs; Paloma Casteleiro Costa; Caroline Filan; Francisco E. Robles

doi:10.1117/12.2650837

16 March 2023 Quantitative oblique back-illumination microscopy with enhanced nuclear phase contrast using acetic acid

Zhe Guang, Amunet Jacobs, Paloma Casteleiro Costa, Caroline Filan, Francisco E. Robles

Proceedings Volume 12389, Quantitative Phase Imaging IX; 1238909 (2023) https://doi.org/10.1117/12.2650837
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Quantitative oblique back-illumination microscopy (qOBM) enables quantitative phase imaging (QPI) in thick samples using epi-illumination. While qOBM offers unprecedented access to refractive index (RI) information in arbitrarily thick scattering samples, QPI-based (or RI index based) imaging still suffers from low cell nuclear contrast, which important for disease detection, including cancer. In this work, we use the acetowhitening effect of acetic acid to enhance the nuclear phase contrast of thick fresh tissue samples. Imaging results from brain samples are presented. Acetic acid phase staining may have important implications for in-vivo QPI-based disease detection

Conference Presentation

Citation Download Citation

Zhe Guang, Amunet Jacobs, Paloma Casteleiro Costa, Caroline Filan, and Francisco E. Robles "Quantitative oblique back-illumination microscopy with enhanced nuclear phase contrast using acetic acid", Proc. SPIE 12389, Quantitative Phase Imaging IX, 1238909 (16 March 2023); https://doi.org/10.1117/12.2650837

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