Label-free quantification of POEGMA-144 polymerization and thermal response via PC-TIR biosensor

Luis Trabucco; Henry Guess; Joshua Chaj Ulloa; Maisha Ahmed; Sean McFadden; Rohini Thevi Guntnur; Nikolay Akimov; Gabriela Romero; Jing Yong Ye

doi:10.1117/12.2650428

16 March 2023 Label-free quantification of POEGMA-144 polymerization and thermal response via PC-TIR biosensor

Luis Trabucco, Henry Guess, Joshua Chaj Ulloa, Maisha Ahmed, Sean McFadden, Rohini Thevi Guntnur, Nikolay Akimov, Gabriela Romero, Jing Yong Ye

Author Affiliations +

Proceedings Volume 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; 123910G (2023) https://doi.org/10.1117/12.2650428
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Label-free quantification of polymerization is of significant importance in today's research as recent advancements in the polymer field have sparked numerous applications in the biomedical industry. Poly-((Ethylene Glycol) Methyl Ether)- Methacrylate (POEGMA) has been recognized as a replacement for PNIPAM for biomedical applications due to various factors such as biocompatibility and thermal response rate. With a rapid thermal response, POEGMA allows for a variety of biomedical applications including a potential drug delivery mechanism. In this study, a Photonic Crystal-Total Internal Reflection (PC-TIR) biosensor has been utilized to monitor POEGMA-144, which has been synthesized via an Atom Transfer Radical Polymerization (ATRP) grafting-from approach. Monitoring the optical resonance of this biosensor, it allows for quantification of the growth of a layer of an initiator on the sensor surface as well as the growth of the POEGMA on top of it. In addition, the PC-TIR sensor further allows real-time analysis of the polymer’s thermal responsiveness in an aqueous environment. At a well-defined, molecular weight specific, Lower Critical Solution Temperature (LCST) of 42°C, the POEGMA-144 polymer conformationally changes, becoming hydrophobic and collapsing after expelling any water between its PEG side chains. Using the PC-TIR biosensor, a definitive polymer collapse was observed at the LCST; similarly, a re-swelling of the polymer was observed as the ambient temperature of the polymer was allowed to cool back to room temperature.

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Luis Trabucco, Henry Guess, Joshua Chaj Ulloa, Maisha Ahmed, Sean McFadden, Rohini Thevi Guntnur, Nikolay Akimov, Gabriela Romero, and Jing Yong Ye "Label-free quantification of POEGMA-144 polymerization and thermal response via PC-TIR biosensor", Proc. SPIE 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, 123910G (16 March 2023); https://doi.org/10.1117/12.2650428

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