Infrared characterization of spike protein of MERS-CoV, SARS-CoV, SARS-CoV-2 and its variants: first steps toward an optical biosensing device

Tiziana Mancini; Annalisa D'Arco; Marta Di Fabrizio; Rosanna Mosetti; Salvatore Macis; Giovanna Tranfo; Giancarlo Della Ventura; Augusto Marcelli; Massimo Petrarca; Stefano Lupi

doi:10.1117/12.2665812

31 May 2023 Infrared characterization of spike protein of MERS-CoV, SARS-CoV, SARS-CoV-2 and its variants: first steps toward an optical biosensing device

Tiziana Mancini, Annalisa D'Arco, Marta Di Fabrizio, Rosanna Mosetti, Salvatore Macis, Giovanna Tranfo, Giancarlo Della Ventura, Augusto Marcelli, Massimo Petrarca, Stefano Lupi

Author Affiliations +

Proceedings Volume 12572, Optical Sensors 2023; 125720D (2023) https://doi.org/10.1117/12.2665812
Event: SPIE Optics + Optoelectronics, 2023, Prague, Czech Republic

Abstract

In this work we introduce the first step toward the development of an Infrared (IR) sensoristic platform aiming to the detection and discrimination of airborne pathogens. SARS-CoV-2 Spike glycoprotein (S) is considered as a biomarker for virus recognition. A comparative spectroscopic analysis is illustrated, studying Spike glycoprotein subunit S1 from three different viruses, MERS-CoV, SARS-CoV and SARS-CoV-2. Moreover, SARS-CoV-2 variants are also investigated. The IR characterization of their S1 secondary conformation was carried out through Attenuated Total Reflection (ATR), analyzing the Amide I absorption band. In addition, pH-dependent conformational changes of SARS-CoV-2 S1 were investigated, too

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Tiziana Mancini, Annalisa D'Arco, Marta Di Fabrizio, Rosanna Mosetti, Salvatore Macis, Giovanna Tranfo, Giancarlo Della Ventura, Augusto Marcelli, Massimo Petrarca, and Stefano Lupi "Infrared characterization of spike protein of MERS-CoV, SARS-CoV, SARS-CoV-2 and its variants: first steps toward an optical biosensing device", Proc. SPIE 12572, Optical Sensors 2023, 125720D (31 May 2023); https://doi.org/10.1117/12.2665812

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