Robust determination of adherent and suspended cancer cell concentration using optical density

Danni Chen; Duli Yu; Liujia Shi; Xiaoliang Guo

doi:10.1117/12.2674151

9 March 2023 Robust determination of adherent and suspended cancer cell concentration using optical density

Danni Chen, Duli Yu, Liujia Shi, Xiaoliang Guo

Proceedings Volume 12600, International Conference on Optoelectronic Materials and Devices (ICOMD 2022); 1260012 (2023) https://doi.org/10.1117/12.2674151
Event: 2022 International Conference on Optoelectronic Materials and Devices, 2022, Chongqing, China

Abstract

Optical density is commonly used as a simple and rapid indirect measurement method to estimate biomass concentration in liquid cultures. However, the object of optical density detection is often algae cells, colonies, and other microorganisms， few studies adopt optical density to quantitatively measure the concentration of cancer cells. In this paper, different liquid media and cancer cells were used to implement a full-wavelength spectral analysis by microplate reader to find the corresponding robust wavelength. According to the experimental results, we suggest measuring cell concentration at nearinfrared wavelengths, such as 850 nm, to facilitate the subsequent unification of protocols applicable to different cell types and culture conditions. Meanwhile, a portable flow cell sensor based on optical density is demonstrated. The calibration curves under various experimental conditions have high regression coefficient R² values, all greater than 0.99, which are expected to be used for online real-time measurement of cell concentration in biological reaction processes. This study provides the feasibility of using the optical density method as a quick and easy way for indirect quantitative measurement of cancer cell concentration.

Citation Download Citation

Danni Chen, Duli Yu, Liujia Shi, and Xiaoliang Guo "Robust determination of adherent and suspended cancer cell concentration using optical density", Proc. SPIE 12600, International Conference on Optoelectronic Materials and Devices (ICOMD 2022), 1260012 (9 March 2023); https://doi.org/10.1117/12.2674151

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