Parallel phase-shifting incoherent digital holography for two-photon holographic microscopy

Naru Yoneda; Xiangyu Quan; Manoj Kumar; Osamu Matoba

doi:10.1117/12.3007932

20 September 2023 Parallel phase-shifting incoherent digital holography for two-photon holographic microscopy

Naru Yoneda, Xiangyu Quan, Manoj Kumar, Osamu Matoba

Proceedings Volume 12608, Biomedical Imaging and Sensing Conference; 1260818 (2023) https://doi.org/10.1117/12.3007932
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

Two-photon holographic microscopy (TPHM) is a powerful technique for stimulating biological cells three-dimensionally. TPHM requires an imaging technique to detect fluorescence lights from the three-dimensionally distributed cells. In this study, parallel phase-shifting incoherent digital holography is applied to TPHM. Owing to the parallel phase-shifting method, the lights from the cells are detected with the frame rate of an image sensor. The feasibility of the proposed method is experimentally verified.

Citation Download Citation

Naru Yoneda, Xiangyu Quan, Manoj Kumar, and Osamu Matoba "Parallel phase-shifting incoherent digital holography for two-photon holographic microscopy", Proc. SPIE 12608, Biomedical Imaging and Sensing Conference, 1260818 (20 September 2023); https://doi.org/10.1117/12.3007932

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