Comparison of acoustic transients with fringe washouts in OCT M-scans after RPE microsecond laser irradiation

Christian Burri; Mylène Amstutz; Leonie Hoffmann; Simon Salzmann; Christoph Meier; Boris Považay; Martin Frenz

doi:10.1117/12.2669477

11 August 2023 Comparison of acoustic transients with fringe washouts in OCT M-scans after RPE microsecond laser irradiation

Christian Burri, Mylène Amstutz, Leonie Hoffmann, Simon Salzmann, Christoph Meier, Boris Považay, Martin Frenz

Author Affiliations +

Proceedings Volume 12627, Translational Biophotonics: Diagnostics and Therapeutics III; 126271L (2023) https://doi.org/10.1117/12.2669477
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

The treatment of retinal pigment epithelium (RPE) related diseases, utilizing minimally invasive laser techniques like selective retina therapy (SRT) is highly demanded. However, due to the strong inter- and intraindividual variability of RPE absorption, as well as optical transmission, laser microsurgery requires reliable real-time feedback-controlled dosimetry (RFD) to prevent unwanted retinal overexposure. The formation of microbubbles around the strong absorbing melanosomes inside RPE cells, has been identified as the leading mechanism of RPE cell damage during low microsecond laser pulse exposure. Their formation and collapse cause measurable optoacoustic (OA) transients. In the presented experiment OA transients are compared to fringe-washouts in simultaneously recorded optical coherence tomography (OCT) M-scans directly following RPE laser irradiation. Ex-vivo porcine RPE-choroid-sclera explants were exposed to laser pulses of 8, 12, 16 and 20 μs duration and pulse energies ranging from 15 to 100 μJ (wavelength: 532 nm, exposure area: 120 × 120 μm²). Simultaneously, time-resolved OCT M-scans were recorded (central wavelength: 840 nm, scan rate: 77 kHz). Post irradiation, RPE cell damage was quantified using a calcein-AM viability assay and correlated with OA transients and fringe-washouts in OCT M-scans. The results show that the detection of fringe-washouts in OCT M-scans linearly scales with OA transients and correctly identifies the destruction of RPE cells. Furthermore, the findings indicate that the optical detection is more sensitive for SRT dosimetry than OA, because OCT reacts to fast dynamic changes of the scattering structure, which possibly are related to minute cell collapses yet indiscernible by OA transients.

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Christian Burri, Mylène Amstutz, Leonie Hoffmann, Simon Salzmann, Christoph Meier, Boris Považay, and Martin Frenz "Comparison of acoustic transients with fringe washouts in OCT M-scans after RPE microsecond laser irradiation", Proc. SPIE 12627, Translational Biophotonics: Diagnostics and Therapeutics III, 126271L (11 August 2023); https://doi.org/10.1117/12.2669477

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KEYWORDS

Optical coherence tomography

Laser irradiation

Pulsed laser operation

Explants

Signal detection

Acoustics

Retina

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