Kilohertz multimodal SLIDE imaging in the visible

C. Stock; T. F. Kutscher; P. Lamminger; S. Karpf

doi:10.1117/12.2670885

9 August 2023 Kilohertz multimodal SLIDE imaging in the visible

C. Stock, T. F. Kutscher, P. Lamminger, S. Karpf

Proceedings Volume 12630, Advances in Microscopic Imaging IV; 126300E (2023) https://doi.org/10.1117/12.2670885
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

The recently introduced high-speed multiphoton microscopy technique of Spectro Temporal Laser Imaging by Diffractive Excitation (SLIDE) provides fluorescence microscopy and fluorescence lifetime imaging (FLIM) at kilohertz frame-rates. Combined with flow cytometry, these speeds enable acquisitions of high resolution microscopy images of cells in flow with high sensitivity at specificity. Until now, SLIDE used infrared laser light for nonlinear two-photon imaging. Here we present SLIDE in the Visible by frequency-doubling of the 1064 nm Fourier Domain Mode Locked (FDML) laser. This new one-photon SLIDE system centered around 532 nm was used to image autofluorescent cells using fluorescence and FLIM imaging, as well as acquiring transmission images for a morphological imaging channel. The high-speed and multi-modal acquisition will permit applications in high-throughput cell analysis and cell sorting applications.

Citation Download Citation

C. Stock, T. F. Kutscher, P. Lamminger, and S. Karpf "Kilohertz multimodal SLIDE imaging in the visible", Proc. SPIE 12630, Advances in Microscopic Imaging IV, 126300E (9 August 2023); https://doi.org/10.1117/12.2670885

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Fluorescence

Fluorescence lifetime imaging

Fluorescence imaging

Second harmonic generation

Laser scanners

Image restoration

Imaging systems

Show All Keywords

Keywords/Phrases

Search In:

Publication Years